| First Author | Lichter P | Year | 1986 |
| Journal | Eur J Biochem | Volume | 160 |
| Issue | 2 | Pages | 419-26 |
| PubMed ID | 3021460 | Mgi Jnum | J:55256 |
| Mgi Id | MGI:1339657 | Doi | 10.1111/j.1432-1033.1986.tb09989.x |
| Citation | Lichter P, et al. (1986) Partial characterization of the human beta-myosin heavy-chain gene which is expressed in heart and skeletal muscle. Eur J Biochem 160(2):419-26 |
| abstractText | A human myosin heavy-chain gene, cloned in gamma Charon 4A phage (and as a clone designated lambda gMHC-1), was shown to code for a cardiac myosin heavy chain of the beta-type. The 5' end of the 14,200-base-pair genomic DNA clone is located in the head region of the myosin chain. The 3' end was shown to extent to the COOH terminus and includes the 3'- nontranslated sequence of the corresponding mRNA. The identification of lambda gMHC-1 as coding for a cardiac beta-myosin heavy chain was achieved by heteroduplex mapping using genomic cardiac myosin heavy- chain DNA of rabbit as a probe and, furthermore, by DNA sequence analysis of three selected subregions of the clones DNA including the 3'-nontranslated sequence. It was demonstrated by the S1 nuclease protection technique that the beta-myosin heavy-chain gene is transcribed in human heart muscle. In addition, we have found by the same technique that it is also expressed in human skeletal muscle. |
