| First Author | Laurila P | Year | 1987 |
| Journal | Somat Cell Mol Genet | Volume | 13 |
| Issue | 1 | Pages | 93-7 |
| PubMed ID | 3027903 | Mgi Jnum | J:8585 |
| Mgi Id | MGI:57051 | Doi | 10.1007/BF02422304 |
| Citation | Laurila P, et al. (1987) Assignment of mouse beta-spectrin gene to chromosome 12. Somat Cell Mol Genet 13(1):93-7 |
| abstractText | The structural gene for the beta-subunit of the mouse erythrocyte spectrin, hereinafter designated as Sp-b, was assigned to the mouse chromosome 12. This assignment was made by Southern analysis of genomic DNA from mouse X Chinese hamster hybrid cells using cloned mouse erythrocyte beta-spectrin cDNA as a probe. In the PstI-digested genomic hamster cell DNA a single band of 2.0 kb was detected, whereas PstI-digested mouse DNA gave a band of 4.2 kb, when probed with the mouse erythroid beta-spectrin cDNA clone. This allowed us to analyze a panel of mouse X Chinese hamster somatic cell hybrids to map this gene to chromosome 12. Interestingly, this assignment is different from that observed for the alpha-subunit of spectrin, which has been mapped to chromosome 1 in mouse. These results serve as a basis for further genetic characterization of the mouse hemolytic anemias. |
