| First Author | Elder PK | Year | 1988 |
| Journal | Mol Cell Biol | Volume | 8 |
| Issue | 1 | Pages | 480-5 |
| PubMed ID | 3422100 | Mgi Jnum | J:8989 |
| Mgi Id | MGI:57453 | Doi | 10.1128/mcb.8.1.480 |
| Citation | Elder PK, et al. (1988) Evidence that the functional beta-actin gene is single copy in most mice and is associated with 5' sequences capable of conferring serum- and cycloheximide-dependent regulation. Mol Cell Biol 8(1):480-5 |
| abstractText | Hybridization to synthetic oligonucleotides representing conserved regions in the promoter and first intron of several vertebrate beta-actin genes was used to discriminate between what appears to be a single functional beta-actin gene and numerous pseudogenes in the mouse genome. Sequences derived from the 5' end of this gene were shown to confer serum-inducible expression upon a heterologous reporter gene when transfected into mouse fibroblasts. Moreover, these sequences rendered reporter gene expression superinducible by a combination of serum and cycloheximide. These experiments indicate that the 5' end of the mouse beta-actin gene contains sequence elements which mediate the stimulatory effects of serum growth factors and which are responsive to both positive and negative regulators of gene expression. |
