| First Author | Baranov VS | Year | 1988 |
| Journal | Biull Eksp Biol Med | Volume | 105 |
| Issue | 5 | Pages | 587-9 |
| PubMed ID | 3132994 | Mgi Jnum | J:9224 |
| Mgi Id | MGI:57687 | Citation | Baranov VS, et al. (1988) [Mapping of apolipoprotein A-1 and ceruloplasmin genes on human, rat and mouse chromosomes by hybridization in situ with specific human DNA probes]. Biull Eksp Biol Med 105(5):587-9 |
| abstractText | In situ hybridization was carried out on metaphase-prometaphase chromosomes of PGA-stimulated lymphocytes and bone marrow cells obtained from laboratory rats and mice. Plasmid cloned sequences of human apolipoprotein A-1 (Apo A-1) and ceruloplasmin (CP) cDNA fragments have been used as specific probes labelled in nick-translation reaction with 3HdTTP and 3Hd ATP. The data of our study suggest that Apo A-1 is localized in 11q14-22, 9 A2-4 and 5q36 areas in men, mice and rats, respectively. The DNA sequences of human CP cDNA most probably occupy 3q23-25, 13q24-26 and 15q13-20 areas. Heterologous in situ hybridization of other species with DNA probes does not always give reliable results in gene mapping. Thus, the data of heterologous hybridization should be considered with caution. |
