| First Author | Tsai-Morris CH | Year | 1988 |
| Journal | Nucleic Acids Res | Volume | 16 |
| Issue | 18 | Pages | 8835-46 |
| PubMed ID | 3140220 | Mgi Jnum | J:9405 |
| Mgi Id | MGI:57866 | Doi | 10.1093/nar/16.18.8835 |
| Citation | Tsai-Morris CH, et al. (1988) 5' flanking sequence and genomic structure of Egr-1, a murine mitogen inducible zinc finger encoding gene. Nucleic Acids Res 16(18):8835-46 |
| abstractText | Egr-1 is a murine zinc finger encoding cDNA whose expression is modulated by a variety of ligand-receptor interactions and is often coregulated with c-fos (1). This study reports the isolation of a mouse Egr-1 genomic clone, its intron-exon structure, and 935 bp of 5' flanking sequence. The gene spans about 3.8 kb and consists of 2 exons and one 700 bp intron. S1 nuclease protection and primer extension analysis were used to define the transcription initiation site. TATA and CCAAT sequences were located at nucleotides -26 and -337 respectively. In addition, there exist five elements whose sequence is nearly identical to the inner core 10 nucleotide region (CCATATTAGG) of the c-fos serum response element, four Sp1 consensus sequences, two AP1 target sequence analogs, and two potential cAMP response elements. These results will ultimately lead to a detailed definition of the intracellular events regulating Egr-1 expression. |
