| First Author | Yohe HC | Year | 1988 |
| Journal | J Leukoc Biol | Volume | 44 |
| Issue | 6 | Pages | 521-8 |
| PubMed ID | 3264007 | Mgi Jnum | J:35993 |
| Mgi Id | MGI:83435 | Doi | 10.1002/jlb.44.6.521 |
| Citation | Yohe HC, et al. (1988) Comparison of thymocyte and T lymphocyte gangliosides from C3H/HeN and C3H/HeJ mice. J Leukoc Biol 44(6):521-8 |
| abstractText | Gangliosides have been prepared from resting murine thymocytes and splenic T cells. Profoundly different two-dimensional thin layer chromatography (2D TLC) patterns were observed between these two cell types. Thymocytes contained 28-30 discrete gangliosides of which eight represented major gangliosides. Splenic T lymphocytes from both strains had much simpler patterns, with six to seven major gangliosides and 12-13 minor gangliosides. Computerized analysis of the thymocyte ganglioside patterns between LPS-responder C3H/HeN mice and lipopolysaccharide (LPS)-hyporesponsive C3H/HeJ mice revealed no significant difference in the major gangliosides. However, with splenic T cell gangliosides, there is a striking difference in the relative proportion of three homologous gangliosides between the two strains. Consistent with previous observations on macrophage gangliosides, the ratio of N-acetylneuraminic acid-containing ganglioside to N-glycolylneuraminic acid-containing ganglioside was higher in both thymocytes and T-cells from the LPS-responder strain. These results show that sialic acid-containing glycolipids from thymocytes and T lymphocytes between endotoxin responder and hyporesponder strains manifest small but significant changes. These differences are present in unstimulated cell populations and may represent a manifestation of the Lps gene. |
