| First Author | Rizzuto R | Year | 1988 |
| Journal | Gene | Volume | 69 |
| Issue | 2 | Pages | 245-56 |
| PubMed ID | 2853101 | Mgi Jnum | J:9661 |
| Mgi Id | MGI:58118 | Doi | 10.1016/0378-1119(88)90435-0 |
| Citation | Rizzuto R, et al. (1988) Subunit Va of human and bovine cytochrome c oxidase is highly conserved. Gene 69(2):245-56 |
| abstractText | We have isolated a full-length cDNA clone specifying the nuclear-encoded subunit Va of the human mitochondrial respiratory enzyme cytochrome c oxidase (COX; EC 1.9.3.1.). The deduced sequence of the polypeptide is 95% identical to that of the corresponding subunit of bovine COX, which makes it the most conserved polypeptide among the known bovine/human pairs of COX subunits. This polypeptide contains an N-terminal presequence which is rich in basic and hydroxylated residues, but differs from the deduced presequences of all other previously isolated COX subunits in that it also contains a negatively charged residue. We find no evidence of tissue-specific isoforms of subunit Va, as Northern analysis showed a single, identically-sized transcript in RNA from human muscle, liver, and brain, while coxVa cDNAs isolated from both endothelial and fetal muscle cDNA libraries had identical nucleotide sequences. |
