| First Author | Carson S | Year | 1989 |
| Journal | Immunogenetics | Volume | 29 |
| Issue | 3 | Pages | 173-9 |
| PubMed ID | 2494106 | Mgi Jnum | J:9664 |
| Mgi Id | MGI:58121 | Doi | 10.1007/BF00373642 |
| Citation | Carson S, et al. (1989) A linkage map of the mouse immunoglobulin lambda light chain locus. Immunogenetics 29(3):173-9 |
| abstractText | The mouse immunoglobulin lambda light chain locus has been linked using field inversion gel electrophoresis. The lambda light chain locus classically contains two V and four J-C gene segments in inbred mouse strains, and was physically mapped in the BALB/c cell line Wehi-3 which contains unrearranged lambda light chain gene segments. The locus is relatively small and spans 300 kb, as defined by a variety of single and double digests using methylation-sensitive restriction enzymes. The order of the lambda gene segments is V2-J2C2J4C4-V1-J3C3J1C1, as was originally proposed. No evidence for nonmethylated CpG rich areas (HTF islands) within the region was found. Fine mapping using the lambda 1, lambda 3 rearranged cell line J558 mapped the gap between the V and J-C gene segments in the lambda 1 gene cluster (V1-J3C3J1C1) to approximately 70 kb. The similar distance (60-100 kb) found in the lambda 2 gene cluster (V2-J2C2J4C4) is further evidence that duplication of an ancestral locus occurred. |
