| First Author | de Vries P | Year | 1989 |
| Journal | J Immunol | Volume | 142 |
| Issue | 8 | Pages | 2841-6 |
| PubMed ID | 2522970 | Mgi Jnum | J:27606 |
| Mgi Id | MGI:75095 | Doi | 10.4049/jimmunol.142.8.2841 |
| Citation | de Vries P, et al. (1989) Measles virus-specific murine T cell clones: characterization of fine specificity and function. J Immunol 142(8):2841-6 |
| abstractText | Measles virus (MV)-specific murine helper T cell clones (Thy-1.2+, CD4+, CD8-) were generated from mice immunized with MV-infected mouse brain homogenate by limiting dilution and in vitro stimulation of spleen cells with UV-inactivated MV Ag. The protein specificity of 7 out of 37 stable T cell clones, which displayed MHC-restricted MV Ag recognition, could be assessed by using purified MV proteins. Two fusion (F) protein-specific, two hemagglutinin-specific, and three nucleoprotein- or matrix protein-specific clones were shown to be established. The F protein-specific T cell clones together with a panel of previously generated F protein-specific T cell clones were characterized for their fine specificity by using beta-galactosidase fusion products, which contained different parts of the F protein. It was shown that at least two epitopes on the major part of the F protein (amino acid 2-513) can be recognized by mouse T cells. Functional characterization of three T cell clones showed that they were able to assist MV-specific B cells and bystander B cells for antibody production. Furthermore, they were shown to produce the lymphokines IL-2 and IFN-gamma. It was also shown that these T cell clones induced a MV-specific delayed type hypersensitivity response. These observations suggest that all of the T cell clones characterized belong to the TH1 helper subset. |
