| First Author | Barberis A | Year | 1990 |
| Journal | Genes Dev | Volume | 4 |
| Issue | 5 | Pages | 849-59 |
| PubMed ID | 2116362 | Mgi Jnum | J:26648 |
| Mgi Id | MGI:74108 | Doi | 10.1101/gad.4.5.849 |
| Citation | Barberis A, et al. (1990) A novel B-cell lineage-specific transcription factor present at early but not late stages of differentiation. Genes Dev 4(5):849-59 |
| abstractText | A novel B-cell-specific transcription factor, BSAP, was identified as a mammalian homolog of the sea urchin protein TSAP, which interacts with the promoters of four tissue-specific late histone H2A-2 and H2B-2 genes. As shown by mobility-shift, methylation interference, and mutational analyses, the mammalian protein BSAP recognizes all four sea urchin binding sites in a manner indistinguishable from TSAP; however, the two proteins differ in molecular weight. BSAP is exclusively restricted to the B-cell lineage of lymphoid differentiation. Its expression appears to be activated during pro-B-cell development, is abundant at the pre-B- and mature B-cell stages, but is absent in terminally differentiated plasma cells. Moreover, BSAP is clearly a B-cell-specific transcription factor, as a wild-type but not a mutant TSAP-binding site of the sea urchin functions only in transfected B cells as an upstream promoter element. Competition experiments did not reveal any high-affinity binding site for BSAP in known regulatory regions of immunoglobulin and class II major histocompatibility (MHC) genes, suggesting that BSAP is a regulator of a different set of B-lymphoid-specific genes. |
