| First Author | Moens PB | Year | 1990 |
| Journal | Cytogenet Cell Genet | Volume | 53 |
| Issue | 4 | Pages | 219-20 |
| PubMed ID | 2209090 | Mgi Jnum | J:10756 |
| Mgi Id | MGI:59202 | Doi | 10.1159/000132935 |
| Citation | Moens PB, et al. (1990) In situ DNA sequence mapping with surface-spread mouse pachytene chromosomes. Cytogenet Cell Genet 53(4):219-20 |
| abstractText | Surface-spread pachytene chromosomes are several times the length of metaphase chromosomes and the decondensed chromatin loops are attached to a well-defined axis (Weith and Traut, 1980). This arrangement permits detailed DNA sequence localization by in situ hybridization. We show that two probes to low-frequency repeated sequences (20 to 50 copies) which locate the centromere proximal in the mouse X metaphase chromosome between bands A1 and A3 (Disteche et al., 1985) and which map 5.5 cM apart (Disteche et al., 1989), hybridize to two distinct chromatin regions 3 to 5 microns apart on a 25 microns long pachytene X chromosome core. |
