| First Author | Martin J | Year | 1990 |
| Journal | J Immunogenet | Volume | 17 |
| Issue | 1-2 | Pages | 151-9 |
| PubMed ID | 2120350 | Mgi Jnum | J:10772 |
| Mgi Id | MGI:59218 | Doi | 10.1111/j.1744-313x.1990.tb00867.x |
| Citation | Martin J, et al. (1990) Expression of I-A alpha k and allelic restriction on A alpha/A beta pairing in transgenic mice. J Immunogenet 17(1-2):151-9 |
| abstractText | Cell surface expression of class 11 major histocompatibility complex encoded (1a) molecules depends on association of the component alpha and beta chain into a stable heterodimer. Studies on cell lines transfected with MHC class II genes have revealed important limitations on the assembly of haplotype-mismatched A alpha:A beta complex. In order to study alpha:beta chain pairing restrictions in vivo a number of lines of transgenic mice carrying the A alpha k gene were generated. The transgene was studied in the context of H-2b, H-2s, H-2q, H-2u, H-2f and H-2v haplotypes. Initial FACS analysis of spleen and peripheral blood cells showed no A alpha kappa expression. Spleen cells stimulated with LPS and analysed by FACS showed A alpha k expression in three different H-2b strains as well as H-2u, but not in others. These data indicate that A alpha k can associate with A beta b and A alpha k, but not with A beta s, A beta q, A beta f, A beta v. |
