| First Author | Bieberich CJ | Year | 1990 |
| Journal | Proc Natl Acad Sci U S A | Volume | 87 |
| Issue | 21 | Pages | 8462-6 |
| PubMed ID | 1978325 | Mgi Jnum | J:30463 |
| Mgi Id | MGI:77973 | Doi | 10.1073/pnas.87.21.8462 |
| Citation | Bieberich CJ, et al. (1990) Evidence for positive and negative regulation of the Hox-3.1 gene. Proc Natl Acad Sci U S A 87(21):8462-6 |
| abstractText | The region-specific patterns of expression of mouse homeobox genes are considered important for establishing the embryonic body plan. A 5-kilobase (kb) DNA fragment from the Hox-3.1 locus that is sufficient to confer region-specific expression to a beta-galactosidase reporter gene in transgenic mouse embryos has been defined. The observed reporter gene expression pattern closely parallels endogenous Hox-3.1 expression in 8- to 9.5-day postcoitum (p.c.) embryos. At 10.5 days p.c. and later, the pattern of beta-galactosidase activity diverges from the Hox-3.1 pattern, and an inappropriately high level of reporter gene expression is observed in posterior spinal ganglia. Inclusion of an additional 2 kb of upstream sequences is sufficient to suppress this aberrant expression in the developing spinal ganglia. Together, these results show that the control of early Hox-3.1 expression is complex, involving at least one positively acting and one negatively acting element. |
