| First Author | Cooke MP | Year | 1991 |
| Journal | Cell | Volume | 65 |
| Issue | 2 | Pages | 281-91 |
| PubMed ID | 2015626 | Mgi Jnum | J:30985 |
| Mgi Id | MGI:78266 | Doi | 10.1016/0092-8674(91)90162-r |
| Citation | Cooke MP, et al. (1991) Regulation of T cell receptor signaling by a src family protein-tyrosine kinase (p59fyn). Cell 65(2):281-91 |
| abstractText | Engagement of the clonotypic antigen receptor (TCR) on T lymphocytes provokes an activation response leading to cell proliferation and lymphokine secretion. To examine the molecular basis of T cell signaling, we generated transgenic animals in which a lymphocyte-specific nonreceptor protein-tyrosine kinase p59fyn(T) is 20-fold overexpressed in developing T lineage cells. Thymocytes from these mice, analyzed using both cellular and biochemical assays, were remarkably hyperstimulable. Moreover, the responsiveness of normal thymocytes to TCR-derived signals correlated well with the extent to which p59fyn was expressed in these cells. Overexpression of a catalytically inactive form of p59fyn substantially inhibited TCR-mediated activation in otherwise normal thymocytes. These effects are unique to p59fyn; overexpression of a closely related T cell-specific tyrosine kinase, p56lck, elicits dramatically different phenotypes. Our results suggest that p59fyn is a critically important component of the TCR signal transduction apparatus. |
