| First Author | Jaspers M | Year | 1991 |
| Journal | Somat Cell Mol Genet | Volume | 17 |
| Issue | 5 | Pages | 505-11 |
| PubMed ID | 1763388 | Mgi Jnum | J:2597 |
| Mgi Id | MGI:51119 | Doi | 10.1007/BF01233174 |
| Citation | Jaspers M, et al. (1991) Localization of the gene encoding the alpha 2 subunit of the human VLA-2 receptor to chromosome 5q23-31. Somat Cell Mol Genet 17(5):505-11 |
| abstractText | The alpha 2 subunit of the VLA-2 receptor (CD49B) was mapped to human chromosome 5 by several independent approaches. First, the expression of the alpha 2 subunit at the protein level was investigated in a panel of human-mouse hybrid cell lines. Cell surface expression was detected by indirect immunofluorescence with monoclonal anti-alpha 2 antibody 12F1. Intracellular alpha 2 antigen was detected by immunostaining of whole cell extracts or of immunoprecipitated 12F1 antigen with the monoclonal antibodies 3H8 and 5C5. Second, the presence of human genomic alpha 2 sequences in the panel of human-mouse hybrids was detected by PCR, using primers derived from the published alpha 2 cDNA sequence. The specificity of the amplification product was shown by direct sequencing. The results of the PCR study were confirmed by amplifying a CD14 gene fragment, known to map to chromosome 5. Finally, in situ hybridization with a 3H-labeled 1040-bp cDNA probe, also obtained by PCR, confirmed and refined the localization of CD49B on chromosome 5 at q23-31. |
