| First Author | Tsuchiya T | Year | 1992 |
| Journal | Biochem Biophys Res Commun | Volume | 182 |
| Issue | 1 | Pages | 137-43 |
| PubMed ID | 1731775 | Mgi Jnum | J:11574 |
| Mgi Id | MGI:59986 | Doi | 10.1016/s0006-291x(05)80122-6 |
| Citation | Tsuchiya T, et al. (1992) In vitro reconstitution of an erythropoietin gene transcription system using its 5'-flanking sequence and a nuclear extract from anemic kidney. Biochem Biophys Res Commun 182(1):137-43 |
| abstractText | We have developed an in vitro transcription system for the erythropoietin (Epo) gene. This system uses a plasmid carrying 0.2 kb of 5'-flanking sequence from the human Epo gene, rNTPs and a nuclear extract from mouse kidney. The transcribed RNA was assayed by primer extension with an end-labeled primer complementary to the sequence of the plasmid, dNTPs and reverse transcriptase. The primer extension product corresponding to the transcript was detected on a sequencing gel. The in vitro promoter activity of the Epo 5'-flanking sequence was observed with a nuclear extract from anemic kidney but not with that from normal kidney. |
