| First Author | D'Amore-Bruno MA | Year | 1992 |
| Journal | Am J Physiol | Volume | 262 |
| Issue | 1 Pt 1 | Pages | L40-7 |
| PubMed ID | 1733280 | Mgi Jnum | J:1959 |
| Mgi Id | MGI:50483 | Doi | 10.1152/ajplung.1992.262.1.L40 |
| Citation | D'Amore-Bruno MA, et al. (1992) Sequence, ontogeny, and cellular localization of murine surfactant protein B mRNA. Am J Physiol 262(1 Pt 1):L40-7 |
| abstractText | We have isolated and characterized murine surfactant protein (SP-B) cDNAs and determined both the cellular distribution and developmental expression of SP-B mRNA. The nucleotide sequence of the composite SP-B cDNAs and cloned genomic SP-B DNA predicted a primary translation product of 377 amino acids (molecular mass = 41.7 kDa) that contained a single asparagine linked glycosylation site. The 410 base 3' untranslated region contained a novel sequence consisting of a (CCA)21 repeat. Tissue specificity and developmental expression of SP-B mRNA was assessed by Northern blot analysis. A single 1.6-kb mRNA species was detected specifically in lung tissue. In fetal mouse lung SP-B mRNA was detected at low levels on day 15 of gestation and increased markedly between days 16 and 17, reaching adult levels by birth. In situ hybridization analysis of SP-B mRNA demonstrated SP-B expression primarily in bronchiolar and alveolar epithelium, although discrete bronchial cells also contained the SP-B mRNA. The murine SP-B mRNA was expressed in proximal and distal epithelial cells within the respiratory tract and shares a close structural relationship to SP-B from other species. |
