| First Author | Geisler C | Year | 1992 |
| Journal | J Immunol | Volume | 148 |
| Issue | 8 | Pages | 2437-45 |
| PubMed ID | 1532815 | Mgi Jnum | J:578 |
| Mgi Id | MGI:49115 | Doi | 10.4049/jimmunol.148.8.2437 |
| Citation | Geisler C (1992) Failure to synthesize the CD3-gamma chain. Consequences for T cell antigen receptor assembly, processing, and expression. J Immunol 148(8):2437-45 |
| abstractText | The TCR consists of the Ti alpha beta heterodimer and the associated CD3 chains, CD3 gamma delta epsilon zeta 2 or zeta eta. The structural relationships between the subunits of the Ti/CD3 complex are still not fully understood. To explore the roles of the individual CD3 chains for the assembly, intracellular processing, and expression of the TCR, mutants of the T cell line Jurkat were isolated. One variant, JGN, was found to produce all the Ti/CD3 components with the exception of CD3-gamma. The results indicate that: 1) the tetrameric form (Ti alpha beta-CD3 delta epsilon) of the Ti/CD3 complex is produced in the endoplasmic reticulum in the absence of CD3-gamma; 2) CD3-zeta does not associate with the Ti alpha beta-CD3 delta epsilon complex; 3) the Ti alpha beta-CD3 delta epsilon complex is not exported from the endoplasmic reticulum to the Golgi apparatus; and 4) CD3-gamma is required for cell surface expression of the Ti/CD3 complex. Transfection of the wild-type CD3-gamma gene into JGN reconstituted expression of functional Ti/CD3 complexes, and analysis of T cell lines producing different amounts of CD3-gamma indicated that CD3-gamma and CD3-delta competed for the binding to CD3-epsilon. |
