| First Author | Annweiler A | Year | 1992 |
| Journal | Nucleic Acids Res | Volume | 20 |
| Issue | 7 | Pages | 1503-9 |
| PubMed ID | 1579442 | Mgi Jnum | J:567 |
| Mgi Id | MGI:49104 | Doi | 10.1093/nar/20.7.1503 |
| Citation | Annweiler A, et al. (1992) Functional analysis of defined mutations in the immunoglobulin heavy-chain enhancer in transgenic mice. Nucleic Acids Res 20(7):1503-9 |
| abstractText | We have analyzed the effect of defined mutations in the mouse immunoglobulin heavy-chain enhancer after introduction into the germline of transgenic mice. We have tested a mutation of the enhancer octamer motif, a double mutation of the octamer motif and the microB-site, and a triple mutation in the microE2, microE3 and microE4-sites. All constructs are expressed in the spleen of transgenic mice. Furthermore, expression is exclusively detectable in lymphoid organs and not in several nonlymphoid tissues. Whereas mutations in the microE-sites have a more pronounced effect on transgene activity in thymocytes as compared to bone marrow and spleen cells, the octamer/microB double mutation shows significantly reduced expression levels only in B-cells. Finally, our results demonstrate that the intronic heavy-chain enhancer element does not contribute to the increase steady state levels of heavy-chain mRNA after stimulation of spleen cells with LPS. |
