First Author | DeAizpurua HJ | Year | 1992 |
Journal | Diabetes | Volume | 41 |
Issue | 9 | Pages | 1182-7 |
PubMed ID | 1499869 | Mgi Jnum | J:37927 |
Mgi Id | MGI:85322 | Doi | 10.2337/diab.41.9.1182 |
Citation | DeAizpurua HJ, et al. (1992) An ELISA for antibodies to recombinant glutamic acid decarboxylase in IDDM. Diabetes 41(9):1182-7 |
abstractText | To detect serum antibodies to GAD in subjects with IDDM, three recombinant mBGAD 67 peptides encompassing the full-length protein were used in an ELISA. In this study 7 of 9 (78%) preclinical IDDM subjects (ICA+ first-degree relatives of a person with IDDM) and 6 of 13 (46%) recent-onset IDDM subjects, but no subjects with Graves' disease (n = 10) or scleroderma (n = 10), nor healthy nondiabetic control subjects (n = 10) had antibodies that reacted with one or more of the recombinant mBGAD peptides. We found no preferential reactivity with any recombinant peptide. Although only 3 preclinical subjects and 1 recent-onset subject had antibodies to all three mBGAD peptides, the results indicate that mBGAD 67 contains at least three B-cell autoepitopes. Compared with an immunoprecipitation assay of native human brain GAD, the ELISA detected 5 of 6 (83%) preclinical and 6 of 6 (100%) recent-onset IDDM subjects. The ELISA should facilitate screening to evaluate the role of autoimmunity to GAD in the development of IDDM. |