| First Author | Mancino F | Year | 1992 |
| Journal | Cytogenet Cell Genet | Volume | 61 |
| Issue | 2 | Pages | 143-5 |
| PubMed ID | 1395725 | Mgi Jnum | J:2895 |
| Mgi Id | MGI:51415 | Doi | 10.1159/000133393 |
| Citation | Mancino F, et al. (1992) Segregation analysis reveals tight genetic linkage between the spontaneously arising neural tube defect gene splotch (Sp) and Pax-3 in an intraspecific mouse backcross. Cytogenet Cell Genet 61(2):143-5 |
| abstractText | Concurrent research has recently characterized Sp2H, a radiation induced mutation at the splotch (Sp) locus, and found alterations in the murine paired box gene, Pax-3, in homozygous Sp2H DNA. It was proposed that Sp and Pax-3 are the same gene. This report presents additional genetic evidence in support of this finding through linkage studies. Southern blot analysis of genomic DNAs from a panel of 125 intraspecific [(Sp/+ x CBA/J)F1-Sp x CBA/J] backcross mice reveals no crossover between Pax-3 and the spontaneously occurring splotch allele, Sp. This positions Pax-3 within 2.9 cM of the Sp locus (95% confidence interval) and suggests tight genetic linkage between the two marker genes. |
