| First Author | Ray D | Year | 1992 |
| Journal | Mol Cell Biol | Volume | 12 |
| Issue | 10 | Pages | 4297-304 |
| PubMed ID | 1406622 | Mgi Jnum | J:2525 |
| Mgi Id | MGI:51047 | Doi | 10.1128/mcb.12.10.4297 |
| Citation | Ray D, et al. (1992) Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1. Mol Cell Biol 12(10):4297-304 |
| abstractText | We have cloned a human cDNA from a new gene, spi-B, on the basis of its homology with the DNA-binding domain of the Spi-1/PU.1 putative oncogene product. spi-B codes for a protein of 262 amino acids presenting 43% overall identity with Spi-1. Its highly basic carboxy-terminal region exhibits 34% sequence identity with the DNA-binding domain of the Ets-1 protein. We showed that the Spi-B protein is able to bind the purine-rich sequence (PU box) recognized by Spi-1/PU.1 and to activate transcription of a reporter plasmid containing PU boxes. Chromosome in situ hybridization allowed us to map spi-B to the 19q13.3-19q13.4 region of the human genome. spi-B, like spi-1, was found to be expressed in various murine and human hematopoietic cell lines except T lymphoid cell lines. |
