| First Author | Sakai K | Year | 1992 |
| Journal | Genomics | Volume | 14 |
| Issue | 1 | Pages | 175-8 |
| PubMed ID | 1427823 | Mgi Jnum | J:2173 |
| Mgi Id | MGI:50697 | Doi | 10.1016/s0888-7543(05)80301-5 |
| Citation | Sakai K, et al. (1992) Molecular cloning and chromosomal mapping of a cDNA encoding human 80K-L protein: major substrate for protein kinase C. Genomics 14(1):175-8 |
| abstractText | We have isolated and sequenced complementary DNA (cDNA) for the human 80K-L protein, a major substrate for protein kinase C and the human homologue of an 80- to 87-kDa bovine protein named MARCKS (myristoylated alanine-rich C kinase substrate). The human 80K-L cDNA encodes a protein of 332 amino acids with a calculated molecular weight of 31,534. Homology comparisons of the nucleotide sequences of the cDNAs indicated that their 3'-untranslated regions are more homologous than the coding regions. Spot blot hybridization using flow-sorted human chromosomes indicated that the gene encoding the 80K-L protein, designated MACS, maps to the q15----qter region of human chromosome 6, and it also suggested that a genomic region with a sequence homologous to the 3'-untranslated region of the 80K-L mRNA exists on chromosome 21. |
