| First Author | Paul E | Year | 1992 |
| Journal | J Immunol | Volume | 149 |
| Issue | 11 | Pages | 3588-95 |
| PubMed ID | 1431128 | Mgi Jnum | J:3434 |
| Mgi Id | MGI:51947 | Doi | 10.4049/jimmunol.149.11.3588 |
| Citation | Paul E, et al. (1992) The anti-DNA-associated idiotype 8.12 is encoded by the V lambda II gene family and maps to the vicinity of L chain CDR1. J Immunol 149(11):3588-95 |
| abstractText | The 8.12 idiotype is an anti-DNA-associated Id present on lambda L chains that are expressed at high titers in 50% of patients with systemic lupus erythematosus. Since this Id can be present on as much as a third of a patient's anti-DNA antibodies and is found in renal glomeruli, 8.12 is thought to be a marker for a subset of pathogenic anti-DNA auto-antibodies. A molecular analysis of the 8.12 positive antibodies was designed to explore the genetic basis of this Id. Monoclonal human B cell lines were generated by transformation with EBV and lambda L chain-secreting lines were analyzed for Id expression and V region gene usage. In this panel of Ig lambda cell lines, the 8.12 idiotype is encoded exclusively by members of the V lambda II gene family. The sequences of several 8.12+ and 8.12- V lambda II genes are reported here and are used to map the 8.12 Id to the vicinity of CDR1, as well as to further characterize the large and polymorphic V lambda II gene family. |
