| First Author | Chen LS | Year | 1992 |
| Journal | Arch Oral Biol | Volume | 37 |
| Issue | 10 | Pages | 771-8 |
| PubMed ID | 1444889 | Mgi Jnum | J:3168 |
| Mgi Id | MGI:51683 | Doi | 10.1016/0003-9969(92)90110-t |
| Citation | Chen LS, et al. (1992) Maintenance of amelogenin gene expression by transformed epithelial cells of mouse enamel organ. Arch Oral Biol 37(10):771-8 |
| abstractText | Electroporation was used to introduce foreign genes into cells derived from the mouse enamel organ epithelia (EOE). Optimal conditions for this electroporation were established. The introduction of a plasmid construct bearing the coding region for the large T-antigen from polyoma virus into EOE cells permitted the establishment of a derivative cell line that has the following characteristics: (1) the cells could be passaged many times; (2) they expressed a keratin-containing cytoskeleton; and (3) approx. 60% of the cells expressed amelogenin, a tissue-specific gene product unique to ameloblasts. Potential uses for such a cell line include analysis of: (1) the upstream regulatory regions required for temporally and spatially restricted expression of amelogenin; (2) the post-translational modification of amelogenin in synchronized cells and (3) the organization and biomineralization of enamel extracellular matrix in monolayer culture. |
