| First Author | Harvey MB | Year | 1992 |
| Journal | Mech Dev | Volume | 38 |
| Issue | 3 | Pages | 169-73 |
| PubMed ID | 1457378 | Mgi Jnum | J:3274 |
| Mgi Id | MGI:51787 | Doi | 10.1016/0925-4773(92)90050-t |
| Citation | Harvey MB, et al. (1992) IGF-2 stimulates growth and metabolism of early mouse embryos. Mech Dev 38(3):169-73 |
| abstractText | Recent reports indicate that the insulin gene family plays a significant role in early development. Both insulin and IGF-1 stimulate growth and metabolism in preimplantation mouse embryos, however, little is known of the physiological effects of IGF-2. In this study, addition of IGF-2 to defined culture medium for the culture of 2-cell embryos stimulated blastocyst formation by 15%, ICM mitogenesis by 37%, and protein synthesis by 35%. EC50s of 12-63 pM IGF-2 for these responses were in the range for mediation by IGF-2 receptors. These results coupled with the previously demonstrated presence and expression of the IGF-2 receptor from the 2-cell stage supports a role for this third member of the insulin gene family in early development. |
