| First Author | Campbell JJ | Year | 1993 |
| Journal | J Immunol | Volume | 150 |
| Issue | 4 | Pages | 1307-13 |
| PubMed ID | 8432979 | Mgi Jnum | J:3929 |
| Mgi Id | MGI:52434 | Doi | 10.4049/jimmunol.150.4.1307 |
| Citation | Campbell JJ, et al. (1993) Recombinase activating gene expression in thymic subpopulations. A transitional cell type has lost RAG-2 but not RAG-1. J Immunol 150(4):1307-13 |
| abstractText | We have used a highly sensitive Northern blot probing technique to examine RAG-1 and RAG-2 mRNA expression in unmanipulated murine thymocytes of various developmental phenotypes sorted by flow cytometry. We have found that neither RAG-1 nor RAG-2 are down-regulated until thymocytes reach the TCRhi stage. TCRhi, CD8+CD4+ cells have lost RAG-2 mRNA but continue to express significant levels of RAG-1 mRNA, providing molecular evidence that this cell type is transitional between the TCRlo, CD4+CD8+ and the TCRhi, CD4+CD8-, or CD4-CD8+ phenotypes. |
