| First Author | Hake LE | Year | 1993 |
| Journal | J Biol Chem | Volume | 268 |
| Issue | 7 | Pages | 4788-97 |
| PubMed ID | 8383125 | Mgi Jnum | J:4083 |
| Mgi Id | MGI:52584 | Doi | 10.1016/s0021-9258(18)53466-9 |
| Citation | Hake LE, et al. (1993) Utilization of an alternative transcription initiation site of somatic cytochrome c in the mouse produces a testis-specific cytochrome c mRNA. J Biol Chem 268(7):4788-97 |
| abstractText | The differential regulation of somatic and testis-specific cytochromes c during spermatogenesis in the mouse is accompanied by changes in mRNA length (Hake, L. E., Alcivar, A. A., and Hecht, N. B. (1990) Development 110, 249-257). In spermatogenic stem cells through early meiotic cells, we detect four somatic cytochrome c (cyt cs) mRNAs of 1.3, 1.1, and 0.7-0.5 kilobases (kb), whereas in postmeiotic cells we detect a larger cyt cs mRNA of 1.7 kb. Oligonucleotide-directed RNase H cleavage of cyt cs mRNA revealed that the 1.7-kb mRNA contains over 1 kb of 5'-untranslated region which is not present in the four shorter cyt cs mRNAs. RNase protection assays indicate that this additional sequence arises from the utilization of an alternative transcription initiation site of the functional cyt cs gene which is 1085 base pairs upstream of the initiation site for the four shorter cyt cs mRNAs. To analyze the promoter for the 1.7-kb mRNA, a genomic clone containing the cyt cs gene and 5 kb of 5'-flanking DNA was isolated. Sequence comparison of the putative promoter region with promoters of other postmeiotically expressed genes reveals several conserved regions. Utilization of this alternative initiation site may be involved in the down-regulation of cytochrome cs during spermatogenesis. |
