| First Author | Thinakaran G | Year | 1993 |
| Journal | FEBS Lett | Volume | 319 |
| Issue | 3 | Pages | 271-6 |
| PubMed ID | 8458421 | Mgi Jnum | J:4632 |
| Mgi Id | MGI:53116 | Doi | 10.1016/0014-5793(93)80561-8 |
| Citation | Thinakaran G, et al. (1993) Expression of c-jun/AP-1 during myogenic differentiation in mouse C2C12 myoblasts. FEBS Lett 319(3):271-6 |
| abstractText | Mitogen withdrawal triggers myogenic differentiation in skeletal myoblasts in culture. We have examined the expression of the proto-oncogene c-jun during this process in mouse C2C12 myoblasts. c-jun belongs to a family of immediate early genes whose expression is activated in cultured cells in response to the addition of serum growth factors. Interestingly, expression of c-jun was maintained in mouse C2C12 and rat L6 myoblasts undergoing myogenic differentiation under low-serum conditions. Previously it has been reported that expression of c-jun is downregulated during differentiation of C2 cells. However, our results using C2C12 cells, a subclone of the C2 line, show that c-jun mRNA, protein and the activator-protein 1 (AP-1) DNA-binding activity were easily detected in proliferating myoblasts and differentiated myotubes. Although overexpression of c-jun has been shown to block myogenic differentiation in C2 cells, results presented here suggest that expression of c-jun at physiological levels may not interfere with skeletal myogenesis. |
