| First Author | Marino LR | Year | 1993 |
| Journal | J Biol Chem | Volume | 268 |
| Issue | 10 | Pages | 7081-9 |
| PubMed ID | 8463242 | Mgi Jnum | J:4392 |
| Mgi Id | MGI:52885 | Doi | 10.1016/s0021-9258(18)53148-3 |
| Citation | Marino LR (1993) Characterization of the mouse carbonic anhydrase II gene promoter. J Biol Chem 268(10):7081-9 |
| abstractText | This work characterizes a 250-base pair (bp) fragment of the mouse carbonic anhydrase II (CAII) gene as an efficient but not cell-specific promoter. This fragment contains multiple consensus cis regulatory regions that interact in a complex fashion to regulate transcriptional activity from the promoter. Truncated fragments of the 250-bp promoter retain transcriptional activity. The 90-bp 5' GC-rich portion of the promoter can direct transcription independently from the consensus TATA box and also contains a silencer that diminishes transcription from the 3' 160-bp portion of the CAII promoter as well as from the SV40 promoter. There are two nonconsensus dual functioning Ap2-like elements on the promoter that are essential for core promoter activity and cAMP-mediated increases in transcription of the gene. A nuclear protein of approximately 65 kDa binds to these elements and is present in nuclear extracts of nonstimulated and forskolin-stimulated NIH-3T3 cells. I conclude that these nonconsensus Ap2-like elements and their cognitive binding protein play a major role in the expression of the CAII gene. |
