| First Author | Heard E | Year | 1993 |
| Journal | Genomics | Volume | 15 |
| Issue | 3 | Pages | 559-69 |
| PubMed ID | 8468050 | Mgi Jnum | J:4231 |
| Mgi Id | MGI:52727 | Doi | 10.1006/geno.1993.1108 |
| Citation | Heard E, et al. (1993) Physical mapping and YAC contig analysis of the region surrounding Xist on the mouse X chromosome. Genomics 15(3):559-69 |
| abstractText | The Xist sequence has been proposed as a potential candidate for the X-inactivation center based both on its localization within the candidate region for the X-inactivation center in man and mouse and on its unique pattern of expression from the inactive X chromosome. We have cloned 550 kb of DNA surrounding the mouse Xist sequence in contiguously overlapping YAC clones and have developed a long-range restriction map that spans almost 1 Mb of this region and includes this YAC contig. The detailed restriction map we have established provides a framework for the identification of expressed sequences other than Xist that may equally exhibit unusual expression characteristics associated with X inactivation. The presence of possible structural or methylation differences within this region between the active and inactive X chromosomes has been investigated through comparative analysis of male and female genomic DNA, and we report here the identification of certain CpG-containing restriction sites around Xist that have an interesting differential methylation status on the inactive and active X chromosomes. |
