| First Author | Chartier FL | Year | 1992 |
| Journal | Nat Genet | Volume | 1 |
| Issue | 2 | Pages | 132-6 |
| PubMed ID | 1302006 | Mgi Jnum | J:1014 |
| Mgi Id | MGI:49546 | Doi | 10.1038/ng0592-132 |
| Citation | Chartier FL, et al. (1992) Construction of a mouse yeast artificial chromosome library in a recombination-deficient strain of yeast. Nat Genet 1(2):132-6 |
| abstractText | We have constructed a new generation yeast artificial chromosome (YAC) library from female C57BL/10 mice in a recombination-deficient strain of Saccharomyces cerevisiae carrying a mutation in the RAD52 gene. The YAC library contains 41,568 clones with an average insert size of 240 kilobases, representing a greater than threefold coverage of the mouse genome. Currently, the library can be screened by polymerase chain reaction and we have isolated positive clones at a number of loci in the mouse genome. This rad52 library should enable a long-term assessment of the effect of one of the yeast recombination pathway genes on both, genome-wide YAC clone stability and the frequency of chimaeric clones. |
