| First Author | Bartels H | Year | 1993 |
| Journal | Histochemistry | Volume | 99 |
| Issue | 4 | Pages | 303-9 |
| PubMed ID | 8500994 | Mgi Jnum | J:13348 |
| Mgi Id | MGI:61549 | Doi | 10.1007/BF00269103 |
| Citation | Bartels H, et al. (1993) Predominant periportal expression of the phosphoenolpyruvate carboxykinase gene in liver of fed and fasted mice, hamsters and rats studied by in situ hybridization. Histochemistry 99(4):303-9 |
| abstractText | Zonal expression of phosphoenolpyruvate carboxykinase (PCK) mRNA in mouse, hamster and rat liver was studied by in situ hybridization with a radiolabelled rat antisense RNA probe. The abundance of PCK mRNA was determined by Northern blot analysis of total RNA with a digoxigenin-labelled probe. Livers were taken from animals that were sacrificed during the normal day/night cycle and after 29 h fasting. In situ hybridization revealed a heterogeneous distribution pattern of PCK mRNA in the liver of all three species throughout the whole day/night cycle. At the end of the dark period, i.e. at the end of feeding, with rats and mice but at a point of continuous feeding with hamsters, low amounts of PCK mRNA were restricted mainly to the periportal area. At the end of the light period, i.e. at the end of fasting with rats and mice but at a point of continuous feeding with hamsters, PCK mRNA levels were increased to a maximum and extended from the periportal to the intermediate zone. In mouse liver prolonged fasting caused a significant increase in PCK mRNA abundance with a nearly homogeneous distribution within the parenchyma. In hamster and rat liver, however, PCK mRNA levels slightly declined or remained constant, respectively, and the predominant localization of PCK mRNA in the periportal and intermediate zone was preserved. The present data suggest that the heterogeneous zonal activation of the PCK gene was essentially very similar in mouse, hamster and rat liver. |
