| First Author | Martinotti MG | Year | 1993 |
| Journal | J Interferon Res | Volume | 13 |
| Issue | 2 | Pages | 105-9 |
| PubMed ID | 8389790 | Mgi Jnum | J:16196 |
| Mgi Id | MGI:64284 | Doi | 10.1089/jir.1993.13.105 |
| Citation | Martinotti MG, et al. (1993) Effect of interferon-alpha on immediate early gene expression of murine cytomegalovirus. J Interferon Res 13(2):105-9 |
| abstractText | Interferon-alpha (IFN-alpha) significantly reduced the replication of murine cytomegalovirus (MCMV) in mouse embryo fibroblasts derived from the susceptible mouse strain C3H/HeJ. When infectious virus production was measured, a strong decrease in virus titer was observed in IFN-treated cells at a multiplicity of infection (moi) of 1 and 0.5 pfu/cell. Analysis of virus-specified mRNAs by Northern blot assay revealed that IFN-alpha had a significant effect on the expression of viral mRNAs at 48h. In particular, the mRNAs of the major immediate early (IE) transcription units, IE1, IE2, and IE3, were impaired by IFN-alpha. In addition, decrease of IE1 mRNA synthesis was accompanied by a reduction of the major IE product (pp89), as revealed by Western blot assay. These results suggest that IFN-alpha may inhibit MCMV replication by directly impairing IE gene transcription. |
