| First Author | Geraghty MT | Year | 1993 |
| Journal | Genomics | Volume | 16 |
| Issue | 2 | Pages | 440-6 |
| PubMed ID | 8314581 | Mgi Jnum | J:43905 |
| Mgi Id | MGI:1099124 | Doi | 10.1006/geno.1993.1208 |
| Citation | Geraghty MT, et al. (1993) The isolation of cDNAs from OATL1 at Xp 11.2 using a 480-kb YAC. Genomics 16(2):440-6 |
| abstractText | Using an ornithine-delta-aminotransferase (OAT) cDNA, we identified five YACs that cover two nonadjacent OAT-related loci in Xp11.2-p11.3, designated OATL1 (distal) and OATL2 (proximal). Because several retinal degenerative disorders map to this region, we used YAC2 (480 kb), which covers the most distal part of OATL1, as a probe to screen a retinal cDNA library. From 8 x 10(4) plaques screened, we isolated 13 clones. Two were OAT cDNAs. The remaining 11 were divided into eight groups by cross-hybridization. Groups 1-4 contain cDNAs that originate from single-copy X-linked genes in YAC2. Each has an open reading frame of > 500 bp and detects one or more transcripts on a Northern blot. The gene for each was sublocalized and ordered in YAC2. The cDNAs in groups 5-8 contained two or more Alu sequences, had no open reading frames, and did not detect transcripts. The cDNAs from groups 1-4 provide expressed sequence tags and identify candidate genes for the genetic disorders that map to this region. |
