| First Author | Beutler B | Year | 1993 |
| Journal | Gene | Volume | 129 |
| Issue | 2 | Pages | 279-83 |
| PubMed ID | 8100792 | Mgi Jnum | J:13317 |
| Mgi Id | MGI:61518 | Doi | 10.1016/0378-1119(93)90280-g |
| Citation | Beutler B, et al. (1993) Polymorphism of the mouse TNF-alpha locus: sequence studies of the 3'-untranslated region and first intron [published erratum appears in Gene 1993 Dec 22;136(1-2):379]. Gene 129(2):279-83 |
| abstractText | We have sequenced the 3'-untranslated region (3'-UTR) and first intron of six strains of Mus musculus, as well as M. spretus, M. castaneus, and M. shanghai. A marked degree of polymorphism was evident in the sequences, and among all of the strains, no two haplotypes were identical. Of particular note, insertional mutations modifying the 5'-TTATTTAT sequence in the 3'-UTR, which in its RNA form acts as a translational repressor, were observed in New Zealand White and M. spretus strains. The mutations that we have identified dictate several restriction-fragment length polymorphisms, which should be useful in following the tumor necrosis factor-encoding gene (TNF) in backcross analysis and in defining the ancestry of TNF in mice of indeterminate parentage. Moreover, the data provide a substrate for the future analysis of differences in the level of TNF expression by genetically distinct mice. |
