| First Author | Vojtek AB | Year | 1993 |
| Journal | Cell | Volume | 74 |
| Issue | 1 | Pages | 205-14 |
| PubMed ID | 8334704 | Mgi Jnum | J:39650 |
| Mgi Id | MGI:87004 | Doi | 10.1016/0092-8674(93)90307-c |
| Citation | Vojtek AB, et al. (1993) Mammalian Ras interacts directly with the serine/threonine kinase Raf. Cell 74(1):205-14 |
| abstractText | We have identified proteins that interact with H-Ras using a two hybrid system screen of a mouse cDNA library. Approximately 50% of the clones identified encoded portions of the c-Raf and A-Raf serine/threonine kinases. Overlaps among these clones define a conserved 81 residue region of the N-terminus of Raf as the Ras interaction region. We show that Raf interacts with wild-type and activated Ras, but not with an effector domain mutant of Ras or with a dominant-interfering Ras mutant. Using purified bacterially expressed fusion proteins, we show, furthermore, that Ras and the N-terminal region of Raf associate directly in vitro and that this interaction is dependent on GTP bound to Ras. |
