| First Author | Clegg DO | Year | 1993 |
| Journal | Gene | Volume | 130 |
| Issue | 2 | Pages | 291-6 |
| PubMed ID | 8395451 | Mgi Jnum | J:14403 |
| Mgi Id | MGI:62572 | Doi | 10.1016/0378-1119(93)90434-5 |
| Citation | Clegg DO (1993) Characterization of a beta-nerve growth factor expression vector for mammalian cells. Gene 130(2):291-6 |
| abstractText | A mammalian expression vector that directs expression of murine beta-nerve growth factor (beta-NGF) from a murine sarcoma virus long terminal repeat (LTR) promoter element was constructed and characterized. The vector, designated pLTRSNGF, was stably transfected into murine L-cells, and beta-NGF mRNA and protein levels were quantified and compared to endogenous levels in control L-cells. Transfection of pLTRSNGF resulted in an approximate doubling of both beta-NGF mRNA and mature beta-NGF protein secreted into the media. Transfection of pLTRSNGF into rat PC 12 cells resulted in colonies of autocrine-differentiating cells that extended dense networks of neurites in the absence of added NGF, indicating that the beta-NGF produced from the vector is biologically active. |
