| First Author | Lehn DA | Year | 1993 |
| Journal | DNA Cell Biol | Volume | 12 |
| Issue | 8 | Pages | 753-61 |
| PubMed ID | 8397832 | Mgi Jnum | J:15440 |
| Mgi Id | MGI:63562 | Doi | 10.1089/dna.1993.12.753 |
| Citation | Lehn DA, et al. (1993) Evolutionarily conserved motifs and protein binding elements in the 5' region of the chromosomal protein HMG-14 gene. DNA Cell Biol 12(8):753-61 |
| abstractText | Although the structure of several genes coding for chromosomal proteins HMG-14 and HMG-17 has been determined, the mechanisms regulating the expression of these genes has not yet been examined. Toward this goal, we have cloned and sequenced a fragment containing the first three exons and 956 bp upstream from the start of translation of the functional mouse HMG-14 gene. Comparison of this sequence to the known sequence of the human HMG-14 gene revealed the presence of five distinct blocks of high sequence identity flanking the start of transcription and the CAAT box. DNase I and mobility-shift analysis identified a DNA region, downstream from the start of transcription, which may be important for the formation of a stable protein-DNA complex. Affinity chromatography on columns containing oligonucleotides corresponding to this sequence indicates that this region is a protein binding site. |
