| First Author | Benvenisty N | Year | 1992 |
| Journal | Genes Dev | Volume | 6 |
| Issue | 12B | Pages | 2513-23 |
| PubMed ID | 1340466 | Mgi Jnum | J:21810 |
| Mgi Id | MGI:69716 | Doi | 10.1101/gad.6.12b.2513 |
| Citation | Benvenisty N, et al. (1992) An embryonically expressed gene is a target for c-Myc regulation via the c-Myc-binding sequence. Genes Dev 6(12B):2513-23 |
| abstractText | We have used a subtraction/coexpression strategy involving two different tumors derived from c-myc-bearing transgenic mice to identify a gene that is a target for c-Myc regulation. The gene, expressed in certain embryonic and adult tissues and in several (but not all) c-myc-based tumors, bears a functional c-Myc-binding sequence located 3' to its transcription start site. This sequence is required for the binding of a nuclear protein complex which, by antibody analysis, includes c-Myc. This site is also required for expression of a reporter gene in chimeric constructs transfected into c-myc-overexpressing cells and, conversely, requires c-myc cotransfection for its enhanced expression in COS cells. Furthermore, transfection of c-myc blocks the normal down-regulation of this gene, which occurs in embryonic stem cells as they undergo differentiation. This target gene encodes an anonymous cDNA (ECA39) found previously to be amplified in a teratocarcinoma cell line. |
