| First Author | Sivo J | Year | 1993 |
| Journal | J Leukoc Biol | Volume | 54 |
| Issue | 5 | Pages | 451-7 |
| PubMed ID | 8228624 | Mgi Jnum | J:15227 |
| Mgi Id | MGI:63356 | Doi | 10.1002/jlb.54.5.451 |
| Citation | Sivo J, et al. (1993) Differential effects of interferon-gamma and glucocorticoids on Fc gamma R gene expression in murine macrophages. J Leukoc Biol 54(5):451-7 |
| abstractText | Interferon-gamma (IFN-gamma) was shown previously to increase Fc gamma receptor (Fc gamma R)-mediated binding and phagocytosis of immunoglobulin G-opsonized erythrocytes in mouse peritoneal exudate macrophages. Glucocorticoids potentiated this effect. We have extended these observations to an investigation of the effects of IFN-gamma and glucocorticoids on steady-state mRNA levels of the three Fc gamma R genes expressed on murine macrophages: Fc gamma RI, Fc gamma RII, and Fc gamma RIII alpha. Fc gamma RI mRNA was present at a barely detectable level in unstimulated cells but was induced 5- to 10-fold by IFN-gamma. Fc gamma RIII alpha mRNA was expressed constitutively and was induced significantly but very modestly (1.5-fold) by IFN-gamma. Fc gamma RII mRNA also exhibited high constitutive expression, but it was not altered by IFN-gamma treatment. Dexamethasone (DEX) significantly increased the expression of IFN-gamma-induced Fc gamma RI mRNA but had no effect on the expression of Fc gamma RII or Fc gamma RIII alpha transcripts in untreated or IFN-gamma-treated cells. The effect of DEX on Fc gamma RI mRNA was seen after about 10 h of simultaneous treatment with IFN-gamma, was dose-dependent, and was glucocorticoid specific. DEX did not modulate the rate of decay of the Fc gamma RI mRNA, suggesting that the up-regulatory effect of DEX may occur, in part, at the level of transcription. In the same culture system, the steady-state level of IFN-gamma-induced Ia mRNA was concurrently inhibited by DEX. The up-regulation of the IFN-gamma-induced high-affinity Fc gamma RI mRNA, the failure to modulate the expression of the two low-affinity Fc gamma R mRNA species, and the down-regulation of IFN-gamma-induced Ia mRNA by DEX in the same cell population illustrate the diverse, gene-specific influence of glucocorticoids on the expression of IFN-gamma-inducible genes. |
