| First Author | Kubo S | Year | 1993 |
| Journal | Gene | Volume | 136 |
| Issue | 1-2 | Pages | 329-31 |
| PubMed ID | 8294025 | Mgi Jnum | J:16477 |
| Mgi Id | MGI:64553 | Doi | 10.1016/0378-1119(93)90489-p |
| Citation | Kubo S, et al. (1993) The gene encoding mouse lymphocyte antigen Ly-49: structural analysis and the 5'-flanking sequence. Gene 136(1-2):329-31 |
| abstractText | Genomic clones encoding the mouse cell-surface antigen, Ly-49, were isolated, and the gene organization was analyzed. The gene spanned approximately 19 kb, and contained seven exons and six introns. The lengths of introns ranged from 1.3 to 8 kb. A 1067-bp sequence in the 5'-flanking region was determined. Primer extension analysis and S1 nuclease mapping revealed a cap site at 158 bp upstream from the ATG coding the N-terminal Met of Ly-49. The 5'-flanking sequence contained a possible promoter sequence, a potential binding site for the T cell-specific transcription factor (TCF-1 alpha/LEF-1), and three sites for the basic helix-loop-helix-binding basic proteins (bHLH). However, no CAAT box-like sequence was present. These results provide important clues for understanding the mechanism of gene expression of lymphocyte antigens. |
