| First Author | Heike T | Year | 1994 |
| Journal | Biochem Biophys Res Commun | Volume | 198 |
| Issue | 2 | Pages | 473-9 |
| PubMed ID | 8297357 | Mgi Jnum | J:16614 |
| Mgi Id | MGI:64682 | Doi | 10.1006/bbrc.1994.1069 |
| Citation | Heike T, et al. (1994) Molecular basis for developmental changes of GM-CSF gene inducibility in embryonal carcinoma cells. Biochem Biophys Res Commun 198(2):473-9 |
| abstractText | In a previous report, we reported that the induction of GM-CSF gene in differentiated P19 cells results from the maturation of the transcriptional machinery. Here, we identified a cis-DNA element which confers the activation of GM-CSF gene in response to PMA/A23187 stimulation in differentiated state. Analysis of the 5'-flanking region between -113 and -60 revealed two elements responsible for promotion and one for inhibition, and the overall effects led to the activation of GM-CSF gene mainly through the sequence between -95 and -73. Using the oligonucleotide between -94 and -73 as a probe in gel retardation assays, we identified a DNA-binding protein, NF-GM-P19, the binding activity of which was induced after differentiation in response to PMA/A23187 stimulation. These results indicate that the induction of GM-CSF gene after differentiation results from the maturation of the transcriptional machinery which recognizes the sequence between -95 and -73. |
