| First Author | Chui D | Year | 1994 |
| Journal | EMBO J | Volume | 13 |
| Issue | 4 | Pages | 798-807 |
| PubMed ID | 7509278 | Mgi Jnum | J:33507 |
| Mgi Id | MGI:80986 | Doi | 10.1002/j.1460-2075.1994.tb06322.x |
| Citation | Chui D, et al. (1994) Specific CD45 isoforms differentially regulate T cell receptor signaling. EMBO J 13(4):798-807 |
| abstractText | Multiple isoforms of T cell CD45 tyrosine phosphatase are expressed as a result of alternative RNA splicing among extracellular exons. To discern the presence and identity of distinct functions among CD45 isoforms, we compared thymic T cell activation responses by elevating expression of two CD45 isoforms normally found on quiescent T cells. We report that CD45RABC significantly increased CD4+ thymic T cell proliferation in both a mixed lymphocyte reaction and following anti-T cell receptor (TCR) antibody stimulation. Additionally, CD45RABC enhanced Ca2+ mobilization and phosphotyrosine accumulation, and suppressed the inhibitory effect of anti-CD4 antibodies. By contrast, CD45R0 did not enhance TCR signaling or phosphotyrosine levels in CD4+ thymic T cells and required a TCR co-stimulus to augment cellular proliferation. These studies provide genetic evidence that alternative CD45 isoforms are functionally distinct and disclose a unique mechanism by which T cell immunologic responsiveness can be modified. |
