| First Author | Norris DP | Year | 1994 |
| Journal | Cell | Volume | 77 |
| Issue | 1 | Pages | 41-51 |
| PubMed ID | 8156596 | Mgi Jnum | J:21454 |
| Mgi Id | MGI:66577 | Doi | 10.1016/0092-8674(94)90233-x |
| Citation | Norris DP, et al. (1994) Evidence that random and imprinted Xist expression is controlled by preemptive methylation. Cell 77(1):41-51 |
| abstractText | The mouse Xist gene is expressed exclusively from the inactive X chromosome and may control the initiation of X inactivation. We show that in somatic tissues the 5' end of the silent Xist allele on the active X chromosome is fully methylated, while the expressed allele on the inactive X is completely unmethylated. In tissues that undergo imprinted paternal Xist expression and imprinted X inactivation, the paternal Xist allele is unmethylated, and the silent maternal allele is fully methylated. In the male germline, a developmentally regulated demethylation of Xist occurs at the onset of meiosis and is retained in mature spermatozoa. This may be the cause of imprinted expression of the paternal Xist allele. A role for methylation in the control of Xist expression is further supported by the finding that in differentiating embryonic stem cells during the initiation of X inactivation, differential methylation of Xist alleles precedes the onset of Xist expression. |
