| First Author | Yu M | Year | 1994 |
| Journal | Gene | Volume | 141 |
| Issue | 2 | Pages | 249-54 |
| PubMed ID | 8163197 | Mgi Jnum | J:18009 |
| Mgi Id | MGI:66031 | Doi | 10.1016/0378-1119(94)90580-0 |
| Citation | Yu M, et al. (1994) A negative regulatory element upstream from the mouse Adh-1 gene can down-regulate a heterologous promoter. Gene 141(2):249-54 |
| abstractText | Regulation of Adh-1 (encoding mouse alcohol dehydrogenase-A2) gene expression results from the interplay of positive and negative effects. We report here the detection and localization of a negative regulatory element (NRE) to the sequence between -754 and -732 bp relative to the transcription start point (tsp). This element, which we call Adh-1 NRE2, can down-regulate transcription from a strong heterologous promoter (the SV40 promoter+enhancer). Adh-1 NRE2 can function in both orientations and either upstream or downstream from the promoter, and thus has attributes characteristic of a 'silencer'. A protein that binds to the Adh-1 NRE is widespread in mouse tissues. |
