| First Author | Mestres P | Year | 1994 |
| Journal | Acta Anat (Basel) | Volume | 149 |
| Issue | 1 | Pages | 13-20 |
| PubMed ID | 8184654 | Mgi Jnum | J:16996 |
| Mgi Id | MGI:65052 | Doi | 10.1159/000147550 |
| Citation | Mestres P, et al. (1994) Expression of casein kinase 2 during mouse embryogenesis. Acta Anat (Basel) 149(1):13-20 |
| abstractText | This paper deals with the expression and distribution of casein kinase 2 (CK-2) subunits in mouse embryos at different developmental stages. Expression was investigated at the mRNA level of CK-2 alpha- and beta-subunits by in situ hybridization and distribution at the protein level by immunohistochemistry using CK-2-alpha- and CK-2-beta-specific antibodies, respectively. In general both methods gave similar results. In earlier stages of mouse embryonic development (day 10.5 after coitus) CK-2 was more expressed in neuroepithelia than in all other tissues. From day 11.5 after coitus on, high expression of CK-2 was detected in all epithelia. From day 16.5 on, all tissues and anlagen of the fetus involved in organogenesis revealed a higher CK-2 expression as compared with secondary mesenchyma. The only difference between in situ hybridization and immunocytochemistry was observed in the skin. Transcripts of CK-2 were found mostly in the basal layer of the epidermis and in the nuclei of keratinocytes, whereas CK-2 protein was almost exclusively found in the cytoplasm of epidermal cells. |
