| First Author | Hatayama T | Year | 1994 |
| Journal | Biochem Biophys Res Commun | Volume | 200 |
| Issue | 3 | Pages | 1367-73 |
| PubMed ID | 8185588 | Mgi Jnum | J:18539 |
| Mgi Id | MGI:66799 | Doi | 10.1006/bbrc.1994.1602 |
| Citation | Hatayama T, et al. (1994) Cellular localization of high-molecular-mass heat shock proteins in murine cells. Biochem Biophys Res Commun 200(3):1367-73 |
| abstractText | We have previously prepared an antiserum raised against hsp105 of murine FM3A cells. The anti-hsp105 serum reacted to two isoforms of high-molecular-mass heat shock protein (HMM-HSP) having a molecular mass of 105,000 Da (hsp105A and hsp105B) in FM3A cells. While hsp105A and hsp105B were both synthesized and accumulated under non-stressed conditions, upon exposure to heat shock much more hsp105B was induced than hsp105A. When the cellular distribution of these hsp105s in murine 3T3 Swiss albino and C3H 10T1/2 cells was examined by immunofluorescence methods using the anti-hsp105 serum, the HMM-HSPs were localized in the cytoplasm and nuclei and were never found in the nucleoli of both 3T3 and C3H cells under non-stressed and stressed conditions. |
