| First Author | Martin KA | Year | 1994 |
| Journal | Gene | Volume | 142 |
| Issue | 2 | Pages | 275-8 |
| PubMed ID | 8194764 | Mgi Jnum | J:18338 |
| Mgi Id | MGI:66338 | Doi | 10.1016/0378-1119(94)90274-7 |
| Citation | Martin KA, et al. (1994) The mouse creatine kinase paired E-box element confers muscle-specific expression to a heterologous promoter. Gene 142(2):275-8 |
| abstractText | E-box elements, with the CANNTG sequence motif, occur in numerous promoters and enhancers. We evaluated the tissue-specific expression properties of the paired murine E-box element from the mouse muscle creatine kinase (MCK) enhancer in a minimal heterologous promoter construct. A 46-bp fragment containing the paired E-box element in its wild-type (wt) configuration conferred high levels of muscle-specific expression in transfected embryonic chicken cell cultures. The expression from this paired E-box element was similar to that of the simian virus 40 (SV40) promoter/enhancer, but a 21-bp fragment containing a single E-box was inactive. We conclude that the paired E-box element from the MCK enhancer is sufficient for high levels of muscle-specific expression when placed upstream from a non-muscle TATA element. |
