| First Author | Jiang X | Year | 1994 |
| Journal | Mamm Genome | Volume | 5 |
| Issue | 3 | Pages | 142-8 |
| PubMed ID | 7911043 | Mgi Jnum | J:17333 |
| Mgi Id | MGI:65380 | Doi | 10.1007/BF00352344 |
| Citation | Jiang X, et al. (1994) The Myb and Ahi-1 genes are physically very closely linked on mouse chromosome 10. Mamm Genome 5(3):142-8 |
| abstractText | Ahi-1 has previously been identified as a common helper provirus integration site on mouse Chromosome (Chr) 10 in 16% of Abelson pre-B-cell lymphomas and shown to be closely linked to the Myb protooncogene. By using long-range restriction mapping, we have mapped the Myb and Ahi-1 regions within a 120-kbp DNA fragment. The Ahi-1 region is located approximately 35 kbp downstream of the Myb gene. A further confirmation of this finding was obtained by screening a mouse YAC library. The three positive clones obtained contained both the Myb and Ahi-1 gene sequences. To test whether provirus integration in the Ahi-1 region enhances the expression of Myb by a cis-acting mechanism, we have also examined Myb gene expression in A-MuLV-induced pre-B-lymphomas. Our data have revealed that there is no clear evidence for such activation in the tumors we have tested, indicating that provirus insertion in the Ahi-1 region is activating a novel gene, apparently involved in tumor formation. |
